The ESETEC^® expression and secretion system developed and patented by Wacker Biotech is based on an E. coli K12 strain and a series of highly expressive plasmids. The E. coli secretion strain can transfer proteins in native conformation across the outer cell membrane into the culture medium. It is extremely stable during fermentation and is routinely employed in commercial-scale production using fermenters with capacities of up to 4 m³. Product yields can already exceed >10 g/h. The ESETEC^® E. coli secretion technology is ideal for antibody fragment production.

Based on proprietary know-how, Wacker Biotech – an expert in the high-cell-density fermentation of E. coli – has developed processes with yields of up to 400 g wet biomass/liter. Additionally, our robust fermentation processes can be validated and ensure high, reproducible yields. The DENSETEC^® high-cell-density fermentation method has already attained yields of more than 10 g/l of active product.

The combination of WACKER’s ESETEC^® secretion technology and DENSETEC^® high-cell-density fermentation accelerates process development and simplifies the purification procedure, thus ensuring that your products are manufactured efficiently and cost-effectively.